8QJP
SmNuc1 nuclease from Stenotrophomonas maltophilia in complex with uridine - 5'- monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.434, 72.800, 82.928 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 43.410 - 1.200 |
| R-factor | 0.1369 |
| Rwork | 0.134 |
| R-free | 0.16690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.560 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.410 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.081 | 1.088 |
| Rmeas | 0.090 | 1.352 |
| Number of reflections | 155893 | 7417 |
| <I/σ(I)> | 11.5 | 1.4 |
| Completeness [%] | 97.0 | 94 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.998 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES pH 6.5, 30 % w/v PEG 8000, 0.2 M Lithium sulfate. Protein concentration 11.8 mg/ml. Cryo-protection by glycerol 20 % v/v. |
