8QJO
SmNuc1 nuclease from Stenotrophomonas maltophilia in complex with guanosine-5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.178, 73.068, 81.607 |
| Unit cell angles | 90.00, 105.32, 90.00 |
Refinement procedure
| Resolution | 41.640 - 1.850 |
| R-factor | 0.1723 |
| Rwork | 0.171 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.445 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.640 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.129 | 1.295 |
| Rmeas | 0.152 | 1.523 |
| Number of reflections | 40335 | 2553 |
| <I/σ(I)> | 7.1 | 1.1 |
| Completeness [%] | 96.4 | 99.3 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.994 | 0.376 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2 M Lithium sulfate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3350, protein concentration 7.5 mg/ml. Cryo-protection by glycerol 20 % v/v. |
