8QJB

T6SS-linked Rhs repeat protein - Salmonella bongori Rhs-core domain extended N-terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.9788
Spacegroup name	P 1 21 1
Unit cell lengths	102.734, 118.733, 130.651
Unit cell angles	90.00, 105.81, 90.00

Refinement procedure

Resolution	47.500 - 3.090
R-factor	0.1874
Rwork	0.185
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Rhs core domain
RMSD bond length	0.002
RMSD bond angle	0.453
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	3.201
High resolution limit [Å]	3.090	3.091
Rmerge	0.122	0.600
Rmeas	0.132	0.651
Rpim	0.050	0.251
Number of reflections	55140	5262
<I/σ(I)>	15.16
Completeness [%]	99.4
Redundancy	6.857
CC(1/2)	0.997	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	Protein solution (1 ul, 4 mg/ml) was mixed with 1 ul reservoir solution (0.1 M MES pH 6.5, 30% ethylene glycol, 7% PEG 8,000) and 0.2 ul of JBScreen Plus compound B9 (2M sodium fluoride)