8QJ4
Receptor Sd-Amt1 (ON-state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 115.098, 115.098, 275.718 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.090 - 1.700 |
| R-factor | 0.15063 |
| Rwork | 0.150 |
| R-free | 0.16615 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.650 | 1.750 |
| Rmerge | 0.106 | 1.693 |
| Rmeas | 0.112 | 1.803 |
| Rpim | 0.035 | 0.614 |
| Total number of observations | 57709 | |
| Number of reflections | 137206 | 6813 |
| <I/σ(I)> | 13.2 | 1.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 10.3 | 8.5 |
| CC(1/2) | 0.999 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis/Tris pH 6.5 with 25 % (w/v) PEG 3350 |
