8QIZ
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H124N soaked with with penta-mannuronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.690, 69.360, 42.920 |
| Unit cell angles | 90.00, 114.64, 90.00 |
Refinement procedure
| Resolution | 39.010 - 1.130 |
| R-factor | 0.1268 |
| Rwork | 0.126 |
| R-free | 0.15850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.044 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4788) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.010 | 1.160 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Number of reflections | 162684 | 4929 |
| <I/σ(I)> | 11.57 | |
| Completeness [%] | 96.9 | |
| Redundancy | 6 | |
| CC(1/2) | 1.000 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 273 | 30% PEG3350, 0.1M Bis-Tris pH 5.5, 0.2M NaCl |
