8QH0
Crystal structure of the SARS-CoV-2 RBD with the antibody Cv2.3194
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9198 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.388, 89.186, 174.471 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.200 - 1.870 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.814 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 1.500 | |
| Rpim | 0.681 | |
| Number of reflections | 70747 | 6950 |
| <I/σ(I)> | 13.2 | 0.96 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 13.2 | |
| CC(1/2) | 0.999 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 20% PEG 3350, 0.2 M lithium citrate |
