8QGW
Crystal structure of oxidized respiratory Complex I subunits NuoEF from Aquifex aeolicus bound to oxidized 3-acetylpyridine adenine dinucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-14 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.9116 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.537, 116.328, 189.621 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.627 - 1.600 |
Rwork | 0.162 |
R-free | 0.18720 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.407 |
Data reduction software | autoPROC |
Data scaling software | Aimless (0.7.9) |
Phasing software | REFMAC (5.8.0352) |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.627 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rpim | 0.033 | 0.429 |
Number of reflections | 185561 | 9111 |
<I/σ(I)> | 14.3 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.4 | 13.9 |
CC(1/2) | 0.999 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | trisodium citrate, ammonium sulfate, sodium chloride, Tris, BisTris |