8QF5
Complex between N-lobe of Arc and nanobody E5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 34.500, 99.480, 138.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.830 - 1.500 |
| R-factor | 0.1457 |
| Rwork | 0.144 |
| R-free | 0.19260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.045 | 2.496 |
| Rmeas | 0.052 | 2.900 |
| Number of reflections | 146561 | 14662 |
| <I/σ(I)> | 10.8 | 0.6 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.8 | |
| CC(1/2) | 0.999 | 0.194 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8, 0.01 M ZnCl2 and 20% w/v PEG 6000 |
