8QEG
Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.604, 102.533, 154.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.253 - 1.700 |
| Rwork | 0.160 |
| R-free | 0.18810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.598 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.350 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 126840 | 6244 |
| <I/σ(I)> | 22.4 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.7 | 14.2 |
| CC(1/2) | 1.000 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.09 M Na Acetate pH 4.5, 2.7 % PEG Smears Medium, 6.3 % MPD, 0.5 n-octyl-beta-D-Glucoside and 3% D-Trehalose |
