8QAM
vaccinia virus Uracil DNA glycosidase mutant I197K-V200E-L204K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97856 |
Spacegroup name | I 4 |
Unit cell lengths | 161.668, 161.668, 39.281 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.200 - 1.320 |
R-factor | 0.13129 |
Rwork | 0.130 |
R-free | 0.16463 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 2.118 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.420 | 1.340 |
High resolution limit [Å] | 1.318 | 1.318 |
Rmerge | 0.050 | 1.134 |
Number of reflections | 119652 | 5311 |
<I/σ(I)> | 23.4 | |
Completeness [%] | 99.5 | 90.2 |
Redundancy | 13.29 | |
CC(1/2) | 0.999 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22 % PEG4000, 100 mM LiSO4, 100 mM Hepes pH 7.5 |