8Q9R
Crystal structure of MADS-box/MEF2D N-terminal domain bound to dsDNA and HDAC9 deacetylase binding motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-11 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.391, 60.042, 77.108 |
Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
Resolution | 47.360 - 2.250 |
R-factor | 0.2251 |
Rwork | 0.223 |
R-free | 0.27270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.746 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.360 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 1.103 | |
Number of reflections | 25008 | 2276 |
<I/σ(I)> | 9 | 1 |
Completeness [%] | 99.7 | |
Redundancy | 3.6 | |
CC(1/2) | 0.998 | 0.372 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM sodium formate, 100 mM Bis-Tris propane pH 6.5 and 20% w/v polyethylene glycol 3350 (PEG 3350) |