8Q9N

Crystal Structure of the MADS-box/MEF2 Domain of MEF2D bound to dsDNA and MITR deacetylase binding motif mutant L151V.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8731
Spacegroup name	P 21 21 21
Unit cell lengths	54.326, 57.000, 76.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.760 - 1.510
R-factor	0.19217
Rwork	0.190
R-free	0.24013
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	1.630
High resolution limit [Å]	1.510	1.600
Rmerge	0.076	0.861
Number of reflections	36328	1562
<I/σ(I)>	12.7	1.9
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM sodium formate, 100 mM Bis-Tris propane pH 6.5 and 20% w/v polyethylene glycol 3350 (PEG 3350)