8Q89

Crystal structure of Apis mellifera glutathione transferase delta 1 in a covalent dimeric state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 43 21 2
Unit cell lengths	77.971, 77.971, 81.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.660 - 2.050
R-factor	0.2187
Rwork	0.217
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.660	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.219	1.017
Rmeas	0.223	1.036
Rpim	0.044	0.198
Number of reflections	16353	1220
<I/σ(I)>	11.3
Completeness [%]	100.0
Redundancy	26.6
CC(1/2)	1.000	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	16% PEG4000, 10% 2-Propanol, 0.1M HEPES pH 7.5, 0.2M Ammonium Sulfate