8Q7I
PCNA from Chaetomium thermophilum in complex with Fen1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-03-20 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.354, 87.354, 214.333 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.860 - 1.950 |
| R-factor | 0.17275 |
| Rwork | 0.171 |
| R-free | 0.20376 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.101 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.860 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.182 |
| Rmeas | 0.074 | 0.193 |
| Rpim | 0.023 | 0.065 |
| Total number of observations | 247082 | 13994 |
| Number of reflections | 23349 | 1631 |
| <I/σ(I)> | 23.8 | 11.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 10.6 | 8.6 |
| CC(1/2) | 0.999 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 50mM Tris-HCl pH8.0, 150mM NaCl |
