8Q5R

HpARI bound to mouse IL-33

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-29
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	149.779, 35.755, 61.289
Unit cell angles	90.00, 92.07, 90.00

Refinement procedure

Resolution	48.260 - 2.100
R-factor	0.1839
Rwork	0.180
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.260	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	19343	1046
<I/σ(I)>	13.1
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.998	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Bis-Tris pH 6.8 and 22% PEG3350