8Q5O

N-terminal domain of restriction endonuclease Eco15I with tetra-methylated target DNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 32 2 1
Unit cell lengths	95.995, 95.995, 96.307
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.998 - 2.330
Rwork	0.264
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.998	2.413
High resolution limit [Å]	2.330	2.330
Rmerge	0.110	1.294
Rmeas	0.113	1.327
Rpim	0.026	0.290
Number of reflections	22349	2202
<I/σ(I)>	20.04	4.33
Completeness [%]	99.8	99.68
Redundancy	19.6	20.6
CC(1/2)	0.999	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M HEPES, pH 6.5, 0.02 M CaCl2, 35 % pentaerythritol ethoxylate