8Q5N

Apo form of restriction endonuclease NhoI.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	38.480, 58.760, 75.590
Unit cell angles	90.00, 97.23, 90.00

Refinement procedure

Resolution	38.170 - 2.650
R-factor	0.2241
Rwork	0.222
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.801
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.250	2.750
High resolution limit [Å]	2.650	2.650
Rmerge	0.113	0.830
Rmeas	0.122	0.900
Rpim	0.047	0.047
Number of reflections	9793	990
<I/σ(I)>	14.57	2.4
Completeness [%]	99.2	99.6
Redundancy	6.7
CC(1/2)	0.997	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	0.1M Tris-BICINE, 20 % v/v Ethylene glycol, 10 % w/v PEG 8000; 0.02 M, 1,6-hexanediol, 0.02 M 1-butanol, 0.02 M 1,2-propanediol, 0.02 M 2-propanol, 0.02 M 1,4-butanediol, 0.02 M 1,3-propanediol