8Q5M
N-terminal domain of restriction endonuclease Eco15I in the absence of DNA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.691, 109.710, 113.952 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.040 - 2.660 |
R-factor | 0.259 |
Rwork | 0.258 |
R-free | 0.28430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.916 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.040 | 2.755 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.038 | 0.898 |
Rmeas | 0.041 | 0.963 |
Rpim | 0.015 | 0.342 |
Number of reflections | 24304 | 2390 |
<I/σ(I)> | 29.39 | 2.08 |
Completeness [%] | 99.8 | 99.92 |
Redundancy | 8 | 7.8 |
CC(1/2) | 1.000 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 3 M NaCl, 0.1 M MES, 10 mM NaSCN as additive |