8Q4I

The crystal structure of human chloride intracellular channel protein 5 delta 57-68

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-02
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.886
Spacegroup name	C 2 2 21
Unit cell lengths	130.128, 220.832, 45.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.560 - 2.100
R-factor	0.2333
Rwork	0.232
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.713
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.023	2.230
High resolution limit [Å]	2.100	2.100
Rmeas	0.054	0.684
Number of reflections	38486	3926
<I/σ(I)>	19.1	1.4
Completeness [%]	99.8
Redundancy	16.7
CC(1/2)	0.999	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 7.0, 30% Polyethylene glycol 3,350