8Q42
Crystal structure of cA4-bound Can2 (E341A) in complex with oligo-A DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-04-07 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.897, 78.026, 94.791 |
| Unit cell angles | 90.00, 95.45, 90.00 |
Refinement procedure
| Resolution | 47.230 - 1.970 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.948 |
| Data reduction software | XDS (20220110) |
| Data scaling software | autoPROC (20230726) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.230 | 2.045 |
| High resolution limit [Å] | 1.970 | 1.974 |
| Rmerge | 0.048 | 0.705 |
| Rmeas | 0.052 | 0.763 |
| Rpim | 0.020 | 0.289 |
| Number of reflections | 48320 | 4786 |
| <I/σ(I)> | 21.6 | 2.59 |
| Completeness [%] | 81.7 | 81.67 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 1.000 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 50 mM Tris pH 8, 17.1% (v/v) PEG 550 MME, 4.3% (w/v) PEG 2000 |
