8Q3M
Structure of Nucleosome Core with a Bound Kaposi Sarcoma Associated Herpesvirus LANA Peptide Having a Methionine to Ornithine Substitution
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2017-09-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.714, 109.882, 184.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.174 - 2.503 |
| Rwork | 0.218 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.515 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.174 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 77598 | 11070 |
| <I/σ(I)> | 23 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.3 | |
| CC(1/2) | 1.000 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Buffers containing MnCl2, KCl and K-cacodylate [pH 6.0] |
