8Q3J

Crystal structure of mIL-38 in complex with a neutralizing Fab e04 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-24
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	73.800, 66.940, 129.050
Unit cell angles	90.00, 104.73, 90.00

Refinement procedure

Resolution	70.110 - 2.500
R-factor	0.2328
Rwork	0.227
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.108
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.110	2.490
High resolution limit [Å]	2.350	2.350
Number of reflections	50677	8129
<I/σ(I)>	16.41
Completeness [%]	99.0	99.2
Redundancy	6.4
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	27.5% (w/v) PEG 1500 100 mM SPG buffer, pH 8.7