8Q3G
Crystal structure of YTHDC1 in complex with Compound 23 (ZA_385)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-05 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.000, 103.670, 42.020 |
Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
Resolution | 38.750 - 1.420 |
R-factor | 0.222 |
Rwork | 0.221 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.927 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.750 | 1.500 |
High resolution limit [Å] | 1.420 | 1.420 |
Number of reflections | 59880 | 9030 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 94.9 | |
Redundancy | 3.36 | |
CC(1/2) | 0.993 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5 |