8Q2D

Crystal structure of the E. coli PqiC Lipoprotein residues 17-187

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-27
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	133.603, 133.603, 65.715
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.730 - 2.080
R-factor	0.2049
Rwork	0.204
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.800
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.730	2.150
High resolution limit [Å]	2.080	2.080
Number of reflections	67192	450
<I/σ(I)>	18.79
Completeness [%]	84.0
Redundancy	2
CC(1/2)	0.999	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	0.1 M sodium chloride, 0.1 M MES; pH 6.5, 33% v/v PEG 400, 4% v/v ethylene glycol