8Q1F
D10N,P146A variant of beta-phosphoglucomutase from Lactococcus lactis in complex with native beta-glucose 1,6-bisphosphate intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-23 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.023, 79.663, 79.848 |
Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
Resolution | 39.830 - 1.010 |
Rwork | 0.115 |
R-free | 0.13480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.486 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.830 | 1.030 |
High resolution limit [Å] | 1.010 | 1.010 |
Rmerge | 0.038 | 0.494 |
Rpim | 0.016 | 0.386 |
Number of reflections | 166355 | 1151 |
<I/σ(I)> | 26 | 0.8 |
Completeness [%] | 80.3 | |
Redundancy | 6.3 | 1.6 |
CC(1/2) | 0.999 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 290 | 0.6 mM bPGM-D10N,P146A 50 mM MgCl2 10 mM bG16BP 28% PEG 4000 100 mM sodium acetate 100 mM tris |