8Q1D

D10N variant of beta-phosphoglucomutase from Lactococcus lactis in complex with fructose 1,6-bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-17
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	31.888, 71.382, 83.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.429 - 1.750
Rwork	0.161
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.567
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.429	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.151	1.330
Rpim	0.043	0.379
Number of reflections	20093	1013
<I/σ(I)>	10.5	1.1
Completeness [%]	100.0	99.2
Redundancy	13.1	13
CC(1/2)	0.998	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	290	0.6 mM bPGM-D10N 5 mM MgCl2 50 mM fructose 1,6-bisphosphate 38 % PEG 4000 200 mM sodium acetate 100 mM tris