8Q0E

Crystal Structure of the N-terminal Domain of Variant Surface Glycoprotein 545 (VSG545) of Trypanosome brucei brucei Lister 427

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	46.414, 52.284, 305.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.520 - 3.150
R-factor	0.2385
Rwork	0.236
R-free	0.28750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.533
Data reduction software	DIALS
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.520	3.260
High resolution limit [Å]	3.150	3.150
Rmerge	0.245
Number of reflections	13575	619
<I/σ(I)>	5.63	1.18
Completeness [%]	75.6	45.3
Redundancy	4.8
CC(1/2)	0.993	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	295	100 mM MES/NaOH 22 % PEG 8000 and 300 mM Li2SO4