8PYH

Crystal structure of the Orange Carotenoid Protein 2 (OCP2) from Crinalium epipsammum PCC 9333

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-14
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 21 21 21
Unit cell lengths	60.054, 103.727, 109.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.200 - 2.200
R-factor	0.17991
Rwork	0.177
R-free	0.22693
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.048
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.200	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.130	0.384
Rmeas	0.140	0.412
Rpim	0.051	0.146
Total number of observations	265057	23129
Number of reflections	35514	2978
<I/σ(I)>	12.3	5.2
Completeness [%]	99.7
Redundancy	7.5	7.8
CC(1/2)	0.993	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate