8PXC
Structure of Fap1, a domain of the accessory Sec-dependent serine-rich glycoprotein adhesin from Streptococcus oralis, solved at wavelength 3.06 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-05-25 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 3.066 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.810, 30.400, 90.600 |
| Unit cell angles | 90.00, 127.04, 90.00 |
Refinement procedure
| Resolution | 65.530 - 1.973 |
| R-factor | 0.18316 |
| Rwork | 0.181 |
| R-free | 0.22042 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.499 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.530 | 2.043 |
| High resolution limit [Å] | 1.973 | 1.973 |
| Rmerge | 0.112 | |
| Rmeas | 0.117 | |
| Rpim | 0.031 | |
| Number of reflections | 17626 | 1321 |
| <I/σ(I)> | 20.29 | 8.67 |
| Completeness [%] | 84.3 | |
| Redundancy | 12.6 | |
| CC(1/2) | 0.990 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M BIS-Tris 5.5 25 % w/v PEG 3350 |
