8PX5
Structure of the RNA recognition motif (RRM) of Seb1 from S. pombe., solved at wavelength 2.75 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-07-04 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.7552 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.100, 46.900, 32.300 |
| Unit cell angles | 90.00, 99.00, 90.00 |
Refinement procedure
| Resolution | 54.870 - 1.770 |
| R-factor | 0.15652 |
| Rwork | 0.155 |
| R-free | 0.18829 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.094 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.870 | 1.838 |
| High resolution limit [Å] | 1.775 | 1.775 |
| Rmerge | 0.045 | 0.252 |
| Rmeas | 0.049 | |
| Rpim | 0.019 | |
| Number of reflections | 10445 | 653 |
| <I/σ(I)> | 22.91 | 3.26 |
| Completeness [%] | 64.9 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.999 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M ammonium formate, 100 mM sodium cacodylate, 8% (w/v) poly-gamma-glutamic acid polymer (PGA-LM, 200-400 kDa low molecular weight polymer) |
