8PWW
PfRH5 bound to monoclonal antibody MAD8-151
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-24 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.95374 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.303, 121.380, 78.998 |
Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
Resolution | 79.000 - 1.945 |
R-factor | 0.2312 |
Rwork | 0.230 |
R-free | 0.25150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Data reduction software | AutoProcess |
Data scaling software | AutoProcess |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.000 | 1.980 |
High resolution limit [Å] | 1.945 | 1.945 |
Number of reflections | 106577 | 5404 |
<I/σ(I)> | 6 | |
Completeness [%] | 98.5 | |
Redundancy | 6.9 | |
CC(1/2) | 0.993 | 0.337 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M sodium chloride, 0.1 M sodium HEPES pH 7 .5, 12% w/v PEG 8000 |