8PRY
Crystal structure of Trypanosoma cruzi glycerol kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.779, 89.321, 97.155 |
| Unit cell angles | 117.35, 88.99, 93.68 |
Refinement procedure
| Resolution | 44.610 - 2.070 |
| R-factor | 0.17899 |
| Rwork | 0.176 |
| R-free | 0.23753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.298 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.610 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 117172 | 4908 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 93.8 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.997 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% w/v Polyethylene glycol 3350, 200 mM Magnesium Formate |
