8PQK
APO crystal structure of PDGFRA-T674I kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.900, 49.020, 76.980 |
| Unit cell angles | 90.00, 100.69, 90.00 |
Refinement procedure
| Resolution | 43.810 - 2.000 |
| R-factor | 0.1974 |
| Rwork | 0.196 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.473 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.120 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.129 | 0.851 |
| Number of reflections | 23810 | 3145 |
| <I/σ(I)> | 9.24 | 2.04 |
| Completeness [%] | 99.3 | 98.1 |
| Redundancy | 6.82 | 6.69 |
| CC(1/2) | 0.997 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 10 mg/mL, 22% PEG3350, 100 mM Bis-Tris-Propane, pH 7.5 |
