8PQ0
Structure of human PARK7 in complex with GK16R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-22 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 66.722, 66.722, 176.528 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.360 - 1.480 |
R-factor | 0.1494 |
Rwork | 0.148 |
R-free | 0.18160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.215 |
Data reduction software | XDS (VERSION Jan 31, 2020 BUILT=20200417) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.360 | 1.530 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.026 | 0.912 |
Rmeas | 0.028 | 0.963 |
Number of reflections | 72940 | 3838 |
<I/σ(I)> | 35.3 | 2.4 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 9.6 | |
CC(1/2) | 1.000 | 0.808 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18.6% (v/v) PEG3350, 170 mM NaNO3 |