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8PQ0

Structure of human PARK7 in complex with GK16R

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X10SA
Synchrotron siteSLS
BeamlineX10SA
Temperature [K]100
Detector technologyPIXEL
Collection date2020-06-22
DetectorDECTRIS EIGER2 X 16M
Wavelength(s)1.000
Spacegroup nameP 65 2 2
Unit cell lengths66.722, 66.722, 176.528
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution33.360 - 1.480
R-factor0.1494
Rwork0.148
R-free0.18160
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.013
RMSD bond angle1.215
Data reduction softwareXDS (VERSION Jan 31, 2020 BUILT=20200417)
Data scaling softwareAimless (0.7.4)
Phasing softwarePHASER (2.8.3)
Refinement softwarePHENIX (1.18.2_3874)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]33.3601.530
High resolution limit [Å]1.4801.480
Rmerge0.0260.912
Rmeas0.0280.963
Number of reflections729403838
<I/σ(I)>35.32.4
Completeness [%]99.499.6
Redundancy9.6
CC(1/2)1.0000.808
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP29318.6% (v/v) PEG3350, 170 mM NaNO3

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