8PPM
IL-12Rb1 neutralizing Fab4, crystal kappa variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-24 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 72.069, 72.069, 163.833 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.410 - 2.030 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.485 |
| Data reduction software | XDS (20220820) |
| Data scaling software | XDS (20220820) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 163.830 | 163.830 | 2.150 |
| High resolution limit [Å] | 2.030 | 6.060 | 2.030 |
| Rmeas | 0.110 | 0.045 | 1.104 |
| Number of reflections | 31735 | 1363 | 4250 |
| <I/σ(I)> | 18.38 | 59.45 | 2.05 |
| Completeness [%] | 97.0 | 99.9 | 81.5 |
| Redundancy | 18.71 | 17.97 | 11.25 |
| CC(1/2) | 0.999 | 0.999 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287 | Fab4 dissociated from target in crystallization drop. |
