8POO
Low resolution structure of inactive conformation of the Ktr cation channel in presence of ATP and c-di-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98013 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 127.795, 143.686, 160.669 |
| Unit cell angles | 90.00, 112.37, 90.00 |
Refinement procedure
| Resolution | 48.000 - 5.770 |
| R-factor | 0.4069 |
| Rwork | 0.405 |
| R-free | 0.42560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.635 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.530 | 6.450 |
| High resolution limit [Å] | 5.770 | 5.770 |
| Rmerge | 0.084 | 1.359 |
| Rmeas | 0.091 | 1.472 |
| Rpim | 0.035 | 0.561 |
| Number of reflections | 15151 | 4157 |
| <I/σ(I)> | 9.6 | 1.4 |
| Completeness [%] | 98.6 | 95.7 |
| Redundancy | 6.9 | 6.8 |
| CC(1/2) | 0.998 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl pH 8.5 700 mM KCl 24% PEG 400 |
