8POO

Low resolution structure of inactive conformation of the Ktr cation channel in presence of ATP and c-di-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98013
Spacegroup name	P 1 21 1
Unit cell lengths	127.795, 143.686, 160.669
Unit cell angles	90.00, 112.37, 90.00

Refinement procedure

Resolution	48.000 - 5.770
R-factor	0.4069
Rwork	0.405
R-free	0.42560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.635
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.530	6.450
High resolution limit [Å]	5.770	5.770
Rmerge	0.084	1.359
Rmeas	0.091	1.472
Rpim	0.035	0.561
Number of reflections	15151	4157
<I/σ(I)>	9.6	1.4
Completeness [%]	98.6	95.7
Redundancy	6.9	6.8
CC(1/2)	0.998	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl pH 8.5 700 mM KCl 24% PEG 400