8POB
Crystal structure of Hen Egg White Lysozyme co-crystallized with 10 mM TbXo4-SO3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.648 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.404, 77.404, 37.602 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.700 - 1.740 |
R-factor | 0.1827 |
Rwork | 0.180 |
R-free | 0.22840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.291 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 1.840 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.074 | 0.855 |
Rpim | 0.028 | 0.184 |
Number of reflections | 12161 | 1719 |
<I/σ(I)> | 0.248 | 0.04 |
Completeness [%] | 99.9 | |
Redundancy | 24.1 | |
CC(1/2) | 0.998 | 0.929 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 950 mM sodium chloride, 100mM sodium acetate pH=4.6. Drops were set by mixing one volume of protein solution at 20mg/ml with one volume of 10mM TbXo4 and with one volume of the well solution. |