8PO6

Structure of Escherichia coli HrpA apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978520
Spacegroup name	P 1 21 1
Unit cell lengths	39.605, 114.897, 94.011
Unit cell angles	90.00, 100.86, 90.00

Refinement procedure

Resolution	29.730 - 2.660
R-factor	0.2086
Rwork	0.206
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.603
Data reduction software	SORTAV (BUILT 20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17rc5_3630)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.328	2.702
High resolution limit [Å]	2.656	2.656
Rmerge	0.082	0.587
Rmeas	0.090	0.669
Rpim	0.035	0.314
Number of reflections	23640	1051
<I/σ(I)>	14.6	2
Completeness [%]	99.0	88.8
Redundancy	6.3	4.3
CC(1/2)	0.998	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1M Hepes 7% PEG 20000