8PNK

Crystal structure of the Ded1p RecA1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.000036
Spacegroup name	P 61
Unit cell lengths	110.000, 110.000, 52.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.630 - 2.200
R-factor	0.1908
Rwork	0.189
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.630	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.049	0.712
Rmeas	0.053	0.781
Rpim	0.020	0.318
Number of reflections	18435	1764
<I/σ(I)>	21.69	2.48
Completeness [%]	99.7	97.4
Redundancy	6.6	5.8
CC(1/2)	1.000	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M HEPES pH 7.0, 30% Jeffamine ED-2001 pH 7.0