8PN0
HEV gt3 P domain in complex with glycan-sensitive nAb p60.12
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.430, 94.480, 91.250 |
Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
Resolution | 47.240 - 2.072 |
R-factor | 0.2276 |
Rwork | 0.226 |
R-free | 0.26080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.240 | 2.146 |
High resolution limit [Å] | 2.072 | 2.072 |
Rmeas | 0.129 | |
Number of reflections | 77331 | 3527 |
<I/σ(I)> | 5.3 | |
Completeness [%] | 84.8 | |
Redundancy | 3.27 | |
CC(1/2) | 0.991 | 0.193 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 25% w/v PEG 4000, 0.2M Calcium chloride and 0.1M Tris pH 8.5 |