8PJH

Crystal structure of human insulin desB30 precursor with an Aspartate-Glycine-Lysine C-peptide in dimer (T2) conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-02-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	I 21 3
Unit cell lengths	78.100, 78.100, 78.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.260 - 1.500
R-factor	0.1723
Rwork	0.172
R-free	0.18160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.141
Data reduction software	HKL-2000 (0.93)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.048	0.768
Number of reflections	72811	1250
<I/σ(I)>	45.7	2.2
Completeness [%]	99.2	100
Redundancy	5.7	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	TBD