8PIA

DNA binding domain of HNF-1A bound to P2-HNF4A promoter DNA variant (P2 -181G>T)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	48.695, 48.752, 215.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.550 - 2.800
R-factor	0.2481
Rwork	0.245
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.631
Data reduction software	XDS
Data scaling software	XSCALE (Jan 10, 2022)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.550	2.870
High resolution limit [Å]	2.800	2.800
Rmeas	0.199
Number of reflections	12346	986
<I/σ(I)>	7.15	0.63
Completeness [%]	92.4	98.3
Redundancy	8.9	9.2
CC(1/2)	0.997	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	0.1 M Citrate, pH 5.0, 20% w/v PEG 6000