8PHY
crystal structure of powdery mildews Blumeria graminis f. sp. tritici AVRPM2 (2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.670, 61.850, 37.160 |
| Unit cell angles | 90.00, 94.14, 90.00 |
Refinement procedure
| Resolution | 37.060 - 1.060 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.449 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (PHENIX) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.060 | 1.100 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Number of reflections | 68774 | 3431 |
| <I/σ(I)> | 10.64 | |
| Completeness [%] | 93.0 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.999 | 0.198 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 20 % w/v Polyethylene glycol 3,350; 100 mM BIS-TRIS propane pH 7.5; 200 mM sodium acetate |
