8PHD

Structure of Human Cdc123 bound to domain 3 of eIF2 gamma and ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	60.462, 126.020, 74.644
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	64.220 - 2.080
R-factor	0.1788
Rwork	0.176
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.441
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.220	2.370
High resolution limit [Å]	2.080	2.080
Rmeas	0.151
Number of reflections	39743	1988
<I/σ(I)>	9.1
Completeness [%]	93.0
Redundancy	6.9
CC(1/2)	0.995	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG3350 0.2 M lithium citrate 1 mM ATP 5 mM MgCl2