8PFP
Crystal structure of WRN helicase domain in complex with ATPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.964, 47.707, 55.021 |
| Unit cell angles | 82.37, 67.15, 63.65 |
Refinement procedure
| Resolution | 26.640 - 1.600 |
| R-factor | 0.2131 |
| Rwork | 0.212 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 50497 | 3742 |
| <I/σ(I)> | 12.8 | 1.14 |
| Completeness [%] | 93.4 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.999 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 % PEG3350 0.2 M Sodium citrate tribasic dihydrate |
