8PFK
RNA structure with 1-methylpseudouridine, C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 41.295, 34.777, 31.685 |
| Unit cell angles | 90.00, 128.07, 90.00 |
Refinement procedure
| Resolution | 23.750 - 1.321 |
| R-factor | 0.1469 |
| Rwork | 0.145 |
| R-free | 0.18320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.750 | 1.368 |
| High resolution limit [Å] | 1.321 | 1.321 |
| Rmerge | 0.016 | 0.335 |
| Rmeas | 0.022 | 0.474 |
| Rpim | 0.016 | 0.335 |
| Number of reflections | 14352 | 315 |
| <I/σ(I)> | 24.01 | 2.11 |
| Completeness [%] | 89.5 | 37.65 |
| Redundancy | 1.9 | 1.1 |
| CC(1/2) | 0.999 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 10 % v/v MPD, 40 mM sodium cacodylate, 12 mM spermine, 80 mM sodium chloride, 12 mM potassium chloride and 20 mM magnesium chloride |
