8PDK
X-ray structure of the Thermus thermophilus PilF-GSPIIB domain in the c-di-GMP bound state
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-14 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 108.138, 108.138, 87.922 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.330 - 2.000 |
R-factor | 0.1791 |
Rwork | 0.178 |
R-free | 0.21240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.088 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.330 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.039 | 0.665 |
Rmeas | 0.043 | 0.743 |
Rpim | 0.019 | 0.331 |
Number of reflections | 25934 | 2624 |
<I/σ(I)> | 29.09 | 2.6 |
Completeness [%] | 100.0 | 99.96 |
Redundancy | 5.2 | |
CC(1/2) | 1.000 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 1.8 M Lithiumsulfate 0.1 M Sodiumacetate pH 5.5 |