8PD6
Crystal structure of the TRIM58 PRY-SPRY domain in complex with TRIM-473
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-22 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 55.284, 55.284, 202.763 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.010 - 1.300 |
| R-factor | 0.14054 |
| Rwork | 0.139 |
| R-free | 0.17750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.913 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.010 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.112 | 0.959 |
| Rmeas | 0.115 | 0.986 |
| Rpim | 0.026 | 0.228 |
| Total number of observations | 42380 | |
| Number of reflections | 46465 | 2274 |
| <I/σ(I)> | 12 | 1.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 18.6 | 18.6 |
| CC(1/2) | 0.999 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 25% (w/v) PEG 3550, 0.2 M MgCl2, 0.1 M HEPES |
