8PCH

CRYSTAL STRUCTURE OF PORCINE CATHEPSIN H DETERMINED AT 2.1 ANGSTROM RESOLUTION: LOCATION OF THE MINI-CHAIN C-TERMINAL CARBOXYL GROUP DEFINES CATHEPSIN H AMINOPEPTIDASE FUNCTION

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	38.442, 68.730, 86.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.198 *
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2act
RMSD bond length	0.012 *
RMSD bond angle	1.530 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MAIN
Refinement software	MAIN

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.110 *
Total number of observations	102384 *
Number of reflections	15946
Completeness [%]	98.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5 *		PROTEIN WAS CRYSTALLIZED USING SITTING DROP VAPOR DIFFUSION METHOD FROM 0.05M NA-ACETATE AND 5% MME PEG 5K BUFFER, PH 4.8. CONCENTRATION OF APPLIED PROTEIN WAS 11 MG/ML., vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	drop	sodium acetate	20 (mM)
3	1	drop	EDTA	1 (mM)
4	1	reservoir	sodium acetate	0.05 (M)
5	1	reservoir	mPEG5000	5 (%)