8PAY

Structure of the E.coli DNA polymerase sliding clamp with a covalently bound peptide 2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	109.858, 66.537, 80.798
Unit cell angles	90.00, 128.52, 90.00

Refinement procedure

Resolution	52.610 - 1.210
R-factor	0.1517
Rwork	0.150
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.172
Data scaling software	XDS
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.610	1.310
High resolution limit [Å]	1.210	1.210
Number of reflections	110085	5493
<I/σ(I)>	21
Completeness [%]	96.0
Redundancy	13.6
CC(1/2)	0.990	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293.15	0.2 M Sodium fluoride 20% PEG 3350, pH 7.3